In a message dated 1/5/2012 12:08:05 A.M. Central Standard Time, [log in to unmask] writes: To readers, Sierra Club's Genetic Engineering Action Team has been following the connection between the honeybee demise and exposure to corn seeds coated with neonicotinoids. Included here is info about the latest research. Laurel Hopwood, Chair, Sierra Club Genetic Engineering Action Team CATCH THE BUZZ - Corn Seed Pesticide Kills Bees Corn Seed Treatment As Lethal As It Gets For Honey Bees All Season Long, And Long After The Season Is Gone. It Just Keeps On Killing. by Alan Harman (EDITED) Frightening new research shows honey bees are being exposed to deadly neonicotinoid insecticides and several other agricultural pesticides throughout their foraging period. The research, published in the scientific journal PLoS One says extremely high levels of clothianidin and thiamethoxam were found in planter exhaust material produced during the planting of treated maize seed. The work, which could raise new questions about the long-term survival of the honey bee, was conducted by Christian H. Krupke of the Department of Entomology at Purdue University, Brian D. Eitzer of the Department of Analytical Chemistry at the Connecticut Agricultural Experiment Station and Krispn Given of Purdue. "Neonicotinoids were found in the soil of each field we sampled, including unplanted fields," they report. Dandelions visited by foraging bees growing near these fields were found to contain neonicotinoids as well. "This indicates deposition of neonicotinoids on the flowers, uptake by the root system, or both. Dead bees collected near hive entrances during the spring sampling period were found to contain clothianidin as well." "These results have implications for a wide range of large-scale annual cropping systems that utilize neonicotinoid seed treatments," the report says. The research was funded by grants from the North American Pollinator Protection Campaign and the Managed Pollinator Coordinated Agricultural Project. Neonicotinoids are persistent. The new report says the half-lives of these compounds in aerobic soil conditions can vary widely, but are best measured in months - 148 - 1,155 days for clothianidin. Among the largest single uses of these compounds is application to maize seed. Production of maize for food, feed and ethanol production represents the largest single use of arable land in North America, reaching a record 88,216,620 acres in 2010 and is expected to increase. All of the maize seed planted in North America except for 0.2% used in organic production is coated with neonicotinoid insecticides. Two major compounds are used - clothianidin and thiamethoxam, with the latter metabolized to clothianidin in the insect. The application rates for these compounds range from 0.25 to 1.25 mg/kernel. These compounds are highly toxic to honey bees - a single kernel contains several orders of magnitude of active ingredient more than the published LD50 values for honey bees - defined as the amount of material that will kill 50% of exposed individuals. In fact, the amount of clothianidin on a single maize seed at the rate of 0.5 mg/kernel contains enough active ingredient to kill more than 80,000 honey bees. The results prompted researchers to carry out more experiments to determine how honey bees may be gaining exposure to clothianidin and other pesticides commonly applied to either maize seed or to plants later in the season. They collected samples from a variety of potential exposure routes near agricultural fields and analyzed them to determine whether pesticides were present. They sampled soils, pollen both collected by honey bees and directly from plants, dandelion flowers, and dead and healthy bees. They even checked waste products produced during the planting of treated seed. Maize seed is sewn with tractor-drawn planters that use a forced air/vacuum system and a perforated disc to pick up individual seeds and drop them into the planting furrow at the selected spacing. Maize kernels treated with neonicotinoids and other compounds such as fungicides do not flow readily and may stick to one another, causing uneven plant spacing. To overcome this, talc (a mineral composed of hydrated magnesium silicate) is added to seed boxes to reduce friction and stickiness and ensure the smooth flow of seed. Much of the talc is exhausted during planting, either down with the seed or behind the planter and into the air using an exhaust fan. Researchers sampled the waste talc after planting to determine whether this material was contaminated with pesticides abraded from treated seeds. The waste is a mixture of the talc that has been in contact with treated maize kernels and minute pieces of the seeds. "Soil collected from areas near our test site revealed that neonicotinoid insecticide residues were present in all samples tested, with clothianidin occurring in each field sampled ... These results demonstrate that honey bees living and foraging near agricultural fields are exposed to neonicotinoids and other pesticides through multiple mechanisms throughout the spring and summer ... We show that bees living in these environments will forage for maize pollen and transport pollen containing neonicotinoids to the hive." The results also showed clothianidin present in the surface soil of fields long after treated seed has been planted. "All soil samples we collected contained clothianidin, even in cases where no treated seed had been planted for two growing seasons," the report says. During the spring planting period, dust that arises from this soil may land on flowers frequented by bees, or possibly on the insects themselves. Of potentially greater concern are the very high levels of neonicotinoids and fungicides found in the talc that has been exposed to treated seed. "The large areas being planted with neonicotinoid treated seeds, combined with the high persistence of these materials and the mobility of disturbed soil and talc dust, carry potential for effects over an area that may exceed the boundaries of the production fields themselves." Later in the season, when planting is largely complete, the researchers found bees collect maize pollen that contains translocated neonicotinoids and other pesticides from seed. Translocation of neonicotinoids into pollen has previously been reported for maize grown from imidacloprid-treated seed, but the researchers say the degree to which honey bees in their study gathered maize pollen was surprising. "The finding that bee-collected pollen contained neonicotinoids is of particular concern because of the risks to newly-emerged nurse bees, which must feed upon pollen reserves in the hive immediately following emergence," they say. "Lethal levels of insecticides in pollen are an obvious concern, but sub-lethal levels are also worthy of study as even slight behavioral effects may impact how affected bees carry out important tasks such as brood rearing, orientation and communication." Also potentially important are the three fungicides found in bee-collected pollen samples - trifloxystrobin and azoxystrobin and propiconazole. Azoxystrobin and trifloxystrobin are frequently used in maize seed treatments as protectants and all three are widely applied to maize in North America, even in the absence of disease symptoms. These findings have implications both for honey bees located near these crops year-round, but also for migratory colonies which pollinate crops such as a lmonds and other fruit and nut crops, the report says. To read the study, click here: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029268 To read our actions on the honeybee demise, click these five sites: http://www.sierraclub.org/biotech/whatsnew/whatsnew_2009-11-10.asp Want to eat? Save the honeybee! http://www.sierraclub.org/biotech/whatsnew/whatsnew_2009-11-09a.asp Sierra Club comments on a "neonic" insecticide http://www.sierraclub.org/biotech/whatsnew/whatsnew_2008-07-30.asp Sierra Club urges EPA to suspend nicotinyl insecticides http://www.sierraclub.org/biotech/whatsnew/whatsnew_2007-04-07.asp USDA, Lobbyists and Bees http://www.sierraclub.org/biotech/whatsnew/whatsnew_2007-03-21.asp GE and bee Colony Collapse Disorder -- science needed! - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - To unsubscribe from the CONS-SPST-BIOTECH-FORUM list, send any message to: [log in to unmask] Check out our Listserv Lists support site for more information: http://www.sierraclub.org/lists/faq.asp - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - To unsubscribe from the IOWA-TOPICS list, send any message to: [log in to unmask] Check out our Listserv Lists support site for more information: http://www.sierraclub.org/lists/faq.asp To view the Sierra Club List Terms & Conditions, see: http://www.sierraclub.org/lists/terms.asp